更新時間:2024-11-03
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F0063 | GST瓊脂糖凝膠4FF/谷胱甘肽瓊脂糖凝膠4FF/Glutathione Sepharose 4FF | BR | 5毫升 | |
10毫升 |
英文名稱:Glutathione Sepharose 4FF
其他名稱:谷胱甘肽瓊脂糖凝膠4FF
級別:BR
Ligand:glutathione and 10-carbon linker arm
Ligand concentration:120-320umol glutathione/ml medium
Binding capacity:~10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity:~11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h)
Mean particle size:90um
Bead structure:highly cross-linked 4% agarose
Maximum flow rate:450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate:Sample loading:<100cm/h(<3ml/min using XK16/20 column);Washing and elution:100~300ml/min using XK16/20 column)
PH stability:3-12
Chemical stability:All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
性狀:含20%乙醇,底部為乳白色膠體
用途:生化研究。專門用于純谷胱甘肽S-轉(zhuǎn)移酶(GST)融合蛋白、其他的谷胱甘肽轉(zhuǎn)移酶以及與谷胱甘肽有親和作用的親和色譜填料,一步分離就可得到很純的目標蛋白,純化條件溫和,可保證蛋白的活性
保存:2~8℃