英文名稱:CM Sephadex C-25 其他名稱:CM葡聚糖凝膠C-25 CAS號(hào):62886-59-3 Type of exchanger:Weak cation exchanger Main functional group:Carboxymethyl Counter ion:Sodium Rec. pH working range:6~10 Mol wt range:up to 30000 Bead form:Spherical,40-125um in dry form Bead structure:Cross-linked dextran Chemical stability:Stable in water,salt solutions, organic solvents,alkaline and weakly acidic solutions and denaturing solvents Physical stability:Robust-have good flow properties and negligible volume changes due to changes in pH and ionic strength. Available capacity(mg/ml avained gel):190mg核糖核酸酶 zui高流速:475cm/h 載量:4-5mmol/g 應(yīng)用:低分子量蛋白、多肽、核苷酸以及巨大分子(MW>20000) 性狀:白色珠狀粉末。是在交聯(lián)葡聚糖凝膠G-25上引入羧甲基的弱酸性陽離子交換劑。 用途:生化研究。它既有離子交換性質(zhì),又有G類葡聚糖凝膠的優(yōu)點(diǎn),如非特異性吸附很少,不易使包括酶在內(nèi)的蛋白質(zhì)變性等。并具有分離大分子量物質(zhì)的特點(diǎn)。使用前需經(jīng)蒸餾水溶脹,稀酸和稀堿等處理,再洗至中性后應(yīng)用。蛋白質(zhì)、多酞、酶、生物堿等的分離 保存:RT,保質(zhì)期5年